protogel8Store and ship at room temperature - No Expensive Dry Ice Shipments ! ProtoGel (30%) 37.5:1 Acrylamide to Bisacrylamide Stabilized Solution; Optimized for SDS-PAGE of Proteins3. Pour Stacking Gel Use ProtoGel Stacking Buffer to make 10 mL of a 4% stacking gel as follows: ProtoGel: 1.33 mL ProtoGel Stacking Buffer: 2.50 mL